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bluejuice gel loading buffer thermo fisher  (Thermo Fisher)


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    Thermo Fisher bluejuice gel loading buffer thermo fisher
    Bluejuice Gel Loading Buffer Thermo Fisher, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bluejuice+gel+loading+buffer+thermo+fisher/CAFFEINE+10GR+10GR/pmc12570318__mmc5-145-209-213
    Average 94 stars, based on 1 article reviews
    bluejuice gel loading buffer thermo fisher - by Bioz Stars, 2026-10
    94/100 stars

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    Recombinant:

    Article Title: High-throughput bioprinting to produce micropatterned neuroepithelial tissues and model TSC2-deficient brain malformations.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-PAX6 Proteintech Group Cat#12323-1-AP; RRID: AB_2159695 Rabbit anti-Ki-67 Proteintech Group Cat#27309-1-AP: RRID: AB_2756525 Mouse anti-ZO-1 Invitrogen Cat#33–9100; RRID: AB_2533147 Mouse anti-β-Tubulin III STEMCELL Technologies Cat# 60100 Mouse anti-N-cad Cell Signaling Cat#14215; RRID: AB_2798427 Chicken anti-MAP2 Millipore Sigma Cat#AB5543; RRID: AB_571049 Mouse anti-S6 Cell Signaling Technology Cat#2317; RRID: AB_2238583 Rabbit anti-Phospho-S6 Cell Signaling Technology Cat#2211S; RRID: AB_331679 IRDye® 800CW Donkey anti-Mouse IgG Secondary Antibody LICORbio Cat#926–32212; RRID: AB_621847 IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody LICORbio Cat#926–68070; RRID: AB_10956588 Chemicals, peptides, and recombinant proteins Rapamycin Cedarlane Cat#13346-10 Accutase STEMCELL Technologies Cat#07922 Hoescht 33342 Enzo Cat#ENZ-52401 DMSO Sigma Cat#D2438 hESC matrigel Corning Cat#354277 DMEM-F12 Gibco Cat#11320-033 Neurobasal Gibco Cat#21103-049 N2 Gibco Cat#17502-048 B27 Gibco Cat#17504-044 MEM Non-Essential Amino Acids Solution (100X) Gibco Cat#11140-050 SB 431542 STEMCELL Technologies Cat#72234 LDN 193189 (2HCl) STEMCELL Technologies Cat#72147 mTeSR plus STEMCELL Technologies Cat#100-0276 HBSS Gibco Cat#14175-095 ReLeSR STEMCELL Technologies Cat#100-0483 ROCK inhibitor STEMCELL Technologies Cat#72304 Prolong antifade ThermoFisher Cat#P36984 Trypan blue STEMCELL Technologies Cat#07050 Cultrex BioTechne Cat#BME001-10 Glutamax Gibco Cat#35050-061 Pierce Protease Inhibitor Tablets ThermoFisher Cat#A32963 Phosphatase Inhibitor Cocktail V Millipore Sigma Cat#524629 RIPA Buffer Sigma-Aldrich Cat#R0278 OneTaq Quick-Load 2X Master Mix New England Biolabs Cat#M0486 PmeI New England Biolabs Cat#R0560 Cutsmart Buffer New England Biolabs Cat#B6004S 10× BlueJuice Gel Loading Buffer Thermo Fisher Cat10816015 Critical commercial assays PowerUp SYBR Green Master Mix Applied Biosystems Cat#A25741 Quick-DNA/RNA Microprep Plus Kit Zymo Research Cat#D7005 MycoFluor Mycoplasma Detection Kit Invitrogen Cat#M7006 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368814 (Continued on next page) e1 Cell Reports Methods 5, 101177, October 20, 2025 OPEN ACCESS .. Human PSC lines used in this study were 168 id2 (iPSC, male) and H1 (hESC, male; WiCell WA01).

    Article Title: High-throughput bioprinting to produce micropatterned neuroepithelial tissues and model TSC2-deficient brain malformations
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-PAX6 Proteintech Group Cat#12323-1-AP; RRID: AB_2159695 Rabbit anti-Ki-67 Proteintech Group Cat#27309-1-AP: RRID: AB_2756525 Mouse anti-ZO-1 Invitrogen Cat#33–9100; RRID: AB_2533147 Mouse anti-β-Tubulin III STEMCELL Technologies Cat# 60100 Mouse anti-N-cad Cell Signaling Cat#14215; RRID: AB_2798427 Chicken anti-MAP2 Millipore Sigma Cat#AB5543; RRID: AB_571049 Mouse anti-S6 Cell Signaling Technology Cat#2317; RRID: AB_2238583 Rabbit anti-Phospho-S6 Cell Signaling Technology Cat#2211S; RRID: AB_331679 IRDye® 800CW Donkey anti-Mouse IgG Secondary Antibody LICORbio Cat#926–32212; RRID: AB_621847 IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody LICORbio Cat#926–68070; RRID: AB_10956588 Chemicals, peptides, and recombinant proteins Rapamycin Cedarlane Cat#13346-10 Accutase STEMCELL Technologies Cat#07922 Hoescht 33342 Enzo Cat#ENZ-52401 DMSO Sigma Cat#D2438 hESC matrigel Corning Cat#354277 DMEM-F12 Gibco Cat#11320-033 Neurobasal Gibco Cat#21103-049 N2 Gibco Cat#17502-048 B27 Gibco Cat#17504-044 MEM Non-Essential Amino Acids Solution (100X) Gibco Cat#11140-050 SB 431542 STEMCELL Technologies Cat#72234 LDN 193189 (2HCl) STEMCELL Technologies Cat#72147 mTeSR plus STEMCELL Technologies Cat#100-0276 HBSS Gibco Cat#14175-095 ReLeSR STEMCELL Technologies Cat#100-0483 ROCK inhibitor STEMCELL Technologies Cat#72304 Prolong antifade ThermoFisher Cat#P36984 Trypan blue STEMCELL Technologies Cat#07050 Cultrex BioTechne Cat#BME001-10 Glutamax Gibco Cat#35050-061 Pierce Protease Inhibitor Tablets ThermoFisher Cat#A32963 Phosphatase Inhibitor Cocktail V Millipore Sigma Cat#524629 RIPA Buffer Sigma-Aldrich Cat#R0278 OneTaq Quick-Load 2X Master Mix New England Biolabs Cat#M0486 PmeI New England Biolabs Cat#R0560 Cutsmart Buffer New England Biolabs Cat#B6004S 10× BlueJuice Gel Loading Buffer Thermo Fisher Cat10816015 Critical commercial assays PowerUp SYBR Green Master Mix Applied Biosystems Cat#A25741 Quick-DNA/RNA Microprep Plus Kit Zymo Research Cat#D7005 MycoFluor Mycoplasma Detection Kit Invitrogen Cat#M7006 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368814 (Continued on next page) e1 Cell Reports Methods 5, 101177, October 20, 2025 Article ll OPEN ACCESS .. Human PSC lines used in this study were 168 id2 (iPSC, male) and H1 (hESC, male; WiCell WA01).

    Protease Inhibitor:

    Article Title: High-throughput bioprinting to produce micropatterned neuroepithelial tissues and model TSC2-deficient brain malformations.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-PAX6 Proteintech Group Cat#12323-1-AP; RRID: AB_2159695 Rabbit anti-Ki-67 Proteintech Group Cat#27309-1-AP: RRID: AB_2756525 Mouse anti-ZO-1 Invitrogen Cat#33–9100; RRID: AB_2533147 Mouse anti-β-Tubulin III STEMCELL Technologies Cat# 60100 Mouse anti-N-cad Cell Signaling Cat#14215; RRID: AB_2798427 Chicken anti-MAP2 Millipore Sigma Cat#AB5543; RRID: AB_571049 Mouse anti-S6 Cell Signaling Technology Cat#2317; RRID: AB_2238583 Rabbit anti-Phospho-S6 Cell Signaling Technology Cat#2211S; RRID: AB_331679 IRDye® 800CW Donkey anti-Mouse IgG Secondary Antibody LICORbio Cat#926–32212; RRID: AB_621847 IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody LICORbio Cat#926–68070; RRID: AB_10956588 Chemicals, peptides, and recombinant proteins Rapamycin Cedarlane Cat#13346-10 Accutase STEMCELL Technologies Cat#07922 Hoescht 33342 Enzo Cat#ENZ-52401 DMSO Sigma Cat#D2438 hESC matrigel Corning Cat#354277 DMEM-F12 Gibco Cat#11320-033 Neurobasal Gibco Cat#21103-049 N2 Gibco Cat#17502-048 B27 Gibco Cat#17504-044 MEM Non-Essential Amino Acids Solution (100X) Gibco Cat#11140-050 SB 431542 STEMCELL Technologies Cat#72234 LDN 193189 (2HCl) STEMCELL Technologies Cat#72147 mTeSR plus STEMCELL Technologies Cat#100-0276 HBSS Gibco Cat#14175-095 ReLeSR STEMCELL Technologies Cat#100-0483 ROCK inhibitor STEMCELL Technologies Cat#72304 Prolong antifade ThermoFisher Cat#P36984 Trypan blue STEMCELL Technologies Cat#07050 Cultrex BioTechne Cat#BME001-10 Glutamax Gibco Cat#35050-061 Pierce Protease Inhibitor Tablets ThermoFisher Cat#A32963 Phosphatase Inhibitor Cocktail V Millipore Sigma Cat#524629 RIPA Buffer Sigma-Aldrich Cat#R0278 OneTaq Quick-Load 2X Master Mix New England Biolabs Cat#M0486 PmeI New England Biolabs Cat#R0560 Cutsmart Buffer New England Biolabs Cat#B6004S 10× BlueJuice Gel Loading Buffer Thermo Fisher Cat10816015 Critical commercial assays PowerUp SYBR Green Master Mix Applied Biosystems Cat#A25741 Quick-DNA/RNA Microprep Plus Kit Zymo Research Cat#D7005 MycoFluor Mycoplasma Detection Kit Invitrogen Cat#M7006 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368814 (Continued on next page) e1 Cell Reports Methods 5, 101177, October 20, 2025 OPEN ACCESS .. Human PSC lines used in this study were 168 id2 (iPSC, male) and H1 (hESC, male; WiCell WA01).

    Article Title: High-throughput bioprinting to produce micropatterned neuroepithelial tissues and model TSC2-deficient brain malformations
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-PAX6 Proteintech Group Cat#12323-1-AP; RRID: AB_2159695 Rabbit anti-Ki-67 Proteintech Group Cat#27309-1-AP: RRID: AB_2756525 Mouse anti-ZO-1 Invitrogen Cat#33–9100; RRID: AB_2533147 Mouse anti-β-Tubulin III STEMCELL Technologies Cat# 60100 Mouse anti-N-cad Cell Signaling Cat#14215; RRID: AB_2798427 Chicken anti-MAP2 Millipore Sigma Cat#AB5543; RRID: AB_571049 Mouse anti-S6 Cell Signaling Technology Cat#2317; RRID: AB_2238583 Rabbit anti-Phospho-S6 Cell Signaling Technology Cat#2211S; RRID: AB_331679 IRDye® 800CW Donkey anti-Mouse IgG Secondary Antibody LICORbio Cat#926–32212; RRID: AB_621847 IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody LICORbio Cat#926–68070; RRID: AB_10956588 Chemicals, peptides, and recombinant proteins Rapamycin Cedarlane Cat#13346-10 Accutase STEMCELL Technologies Cat#07922 Hoescht 33342 Enzo Cat#ENZ-52401 DMSO Sigma Cat#D2438 hESC matrigel Corning Cat#354277 DMEM-F12 Gibco Cat#11320-033 Neurobasal Gibco Cat#21103-049 N2 Gibco Cat#17502-048 B27 Gibco Cat#17504-044 MEM Non-Essential Amino Acids Solution (100X) Gibco Cat#11140-050 SB 431542 STEMCELL Technologies Cat#72234 LDN 193189 (2HCl) STEMCELL Technologies Cat#72147 mTeSR plus STEMCELL Technologies Cat#100-0276 HBSS Gibco Cat#14175-095 ReLeSR STEMCELL Technologies Cat#100-0483 ROCK inhibitor STEMCELL Technologies Cat#72304 Prolong antifade ThermoFisher Cat#P36984 Trypan blue STEMCELL Technologies Cat#07050 Cultrex BioTechne Cat#BME001-10 Glutamax Gibco Cat#35050-061 Pierce Protease Inhibitor Tablets ThermoFisher Cat#A32963 Phosphatase Inhibitor Cocktail V Millipore Sigma Cat#524629 RIPA Buffer Sigma-Aldrich Cat#R0278 OneTaq Quick-Load 2X Master Mix New England Biolabs Cat#M0486 PmeI New England Biolabs Cat#R0560 Cutsmart Buffer New England Biolabs Cat#B6004S 10× BlueJuice Gel Loading Buffer Thermo Fisher Cat10816015 Critical commercial assays PowerUp SYBR Green Master Mix Applied Biosystems Cat#A25741 Quick-DNA/RNA Microprep Plus Kit Zymo Research Cat#D7005 MycoFluor Mycoplasma Detection Kit Invitrogen Cat#M7006 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368814 (Continued on next page) e1 Cell Reports Methods 5, 101177, October 20, 2025 Article ll OPEN ACCESS .. Human PSC lines used in this study were 168 id2 (iPSC, male) and H1 (hESC, male; WiCell WA01).

    SYBR Green Assay:

    Article Title: High-throughput bioprinting to produce micropatterned neuroepithelial tissues and model TSC2-deficient brain malformations.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-PAX6 Proteintech Group Cat#12323-1-AP; RRID: AB_2159695 Rabbit anti-Ki-67 Proteintech Group Cat#27309-1-AP: RRID: AB_2756525 Mouse anti-ZO-1 Invitrogen Cat#33–9100; RRID: AB_2533147 Mouse anti-β-Tubulin III STEMCELL Technologies Cat# 60100 Mouse anti-N-cad Cell Signaling Cat#14215; RRID: AB_2798427 Chicken anti-MAP2 Millipore Sigma Cat#AB5543; RRID: AB_571049 Mouse anti-S6 Cell Signaling Technology Cat#2317; RRID: AB_2238583 Rabbit anti-Phospho-S6 Cell Signaling Technology Cat#2211S; RRID: AB_331679 IRDye® 800CW Donkey anti-Mouse IgG Secondary Antibody LICORbio Cat#926–32212; RRID: AB_621847 IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody LICORbio Cat#926–68070; RRID: AB_10956588 Chemicals, peptides, and recombinant proteins Rapamycin Cedarlane Cat#13346-10 Accutase STEMCELL Technologies Cat#07922 Hoescht 33342 Enzo Cat#ENZ-52401 DMSO Sigma Cat#D2438 hESC matrigel Corning Cat#354277 DMEM-F12 Gibco Cat#11320-033 Neurobasal Gibco Cat#21103-049 N2 Gibco Cat#17502-048 B27 Gibco Cat#17504-044 MEM Non-Essential Amino Acids Solution (100X) Gibco Cat#11140-050 SB 431542 STEMCELL Technologies Cat#72234 LDN 193189 (2HCl) STEMCELL Technologies Cat#72147 mTeSR plus STEMCELL Technologies Cat#100-0276 HBSS Gibco Cat#14175-095 ReLeSR STEMCELL Technologies Cat#100-0483 ROCK inhibitor STEMCELL Technologies Cat#72304 Prolong antifade ThermoFisher Cat#P36984 Trypan blue STEMCELL Technologies Cat#07050 Cultrex BioTechne Cat#BME001-10 Glutamax Gibco Cat#35050-061 Pierce Protease Inhibitor Tablets ThermoFisher Cat#A32963 Phosphatase Inhibitor Cocktail V Millipore Sigma Cat#524629 RIPA Buffer Sigma-Aldrich Cat#R0278 OneTaq Quick-Load 2X Master Mix New England Biolabs Cat#M0486 PmeI New England Biolabs Cat#R0560 Cutsmart Buffer New England Biolabs Cat#B6004S 10× BlueJuice Gel Loading Buffer Thermo Fisher Cat10816015 Critical commercial assays PowerUp SYBR Green Master Mix Applied Biosystems Cat#A25741 Quick-DNA/RNA Microprep Plus Kit Zymo Research Cat#D7005 MycoFluor Mycoplasma Detection Kit Invitrogen Cat#M7006 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368814 (Continued on next page) e1 Cell Reports Methods 5, 101177, October 20, 2025 OPEN ACCESS .. Human PSC lines used in this study were 168 id2 (iPSC, male) and H1 (hESC, male; WiCell WA01).

    Article Title: High-throughput bioprinting to produce micropatterned neuroepithelial tissues and model TSC2-deficient brain malformations
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-PAX6 Proteintech Group Cat#12323-1-AP; RRID: AB_2159695 Rabbit anti-Ki-67 Proteintech Group Cat#27309-1-AP: RRID: AB_2756525 Mouse anti-ZO-1 Invitrogen Cat#33–9100; RRID: AB_2533147 Mouse anti-β-Tubulin III STEMCELL Technologies Cat# 60100 Mouse anti-N-cad Cell Signaling Cat#14215; RRID: AB_2798427 Chicken anti-MAP2 Millipore Sigma Cat#AB5543; RRID: AB_571049 Mouse anti-S6 Cell Signaling Technology Cat#2317; RRID: AB_2238583 Rabbit anti-Phospho-S6 Cell Signaling Technology Cat#2211S; RRID: AB_331679 IRDye® 800CW Donkey anti-Mouse IgG Secondary Antibody LICORbio Cat#926–32212; RRID: AB_621847 IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody LICORbio Cat#926–68070; RRID: AB_10956588 Chemicals, peptides, and recombinant proteins Rapamycin Cedarlane Cat#13346-10 Accutase STEMCELL Technologies Cat#07922 Hoescht 33342 Enzo Cat#ENZ-52401 DMSO Sigma Cat#D2438 hESC matrigel Corning Cat#354277 DMEM-F12 Gibco Cat#11320-033 Neurobasal Gibco Cat#21103-049 N2 Gibco Cat#17502-048 B27 Gibco Cat#17504-044 MEM Non-Essential Amino Acids Solution (100X) Gibco Cat#11140-050 SB 431542 STEMCELL Technologies Cat#72234 LDN 193189 (2HCl) STEMCELL Technologies Cat#72147 mTeSR plus STEMCELL Technologies Cat#100-0276 HBSS Gibco Cat#14175-095 ReLeSR STEMCELL Technologies Cat#100-0483 ROCK inhibitor STEMCELL Technologies Cat#72304 Prolong antifade ThermoFisher Cat#P36984 Trypan blue STEMCELL Technologies Cat#07050 Cultrex BioTechne Cat#BME001-10 Glutamax Gibco Cat#35050-061 Pierce Protease Inhibitor Tablets ThermoFisher Cat#A32963 Phosphatase Inhibitor Cocktail V Millipore Sigma Cat#524629 RIPA Buffer Sigma-Aldrich Cat#R0278 OneTaq Quick-Load 2X Master Mix New England Biolabs Cat#M0486 PmeI New England Biolabs Cat#R0560 Cutsmart Buffer New England Biolabs Cat#B6004S 10× BlueJuice Gel Loading Buffer Thermo Fisher Cat10816015 Critical commercial assays PowerUp SYBR Green Master Mix Applied Biosystems Cat#A25741 Quick-DNA/RNA Microprep Plus Kit Zymo Research Cat#D7005 MycoFluor Mycoplasma Detection Kit Invitrogen Cat#M7006 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368814 (Continued on next page) e1 Cell Reports Methods 5, 101177, October 20, 2025 Article ll OPEN ACCESS .. Human PSC lines used in this study were 168 id2 (iPSC, male) and H1 (hESC, male; WiCell WA01).

    Reverse Transcription:

    Article Title: High-throughput bioprinting to produce micropatterned neuroepithelial tissues and model TSC2-deficient brain malformations.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-PAX6 Proteintech Group Cat#12323-1-AP; RRID: AB_2159695 Rabbit anti-Ki-67 Proteintech Group Cat#27309-1-AP: RRID: AB_2756525 Mouse anti-ZO-1 Invitrogen Cat#33–9100; RRID: AB_2533147 Mouse anti-β-Tubulin III STEMCELL Technologies Cat# 60100 Mouse anti-N-cad Cell Signaling Cat#14215; RRID: AB_2798427 Chicken anti-MAP2 Millipore Sigma Cat#AB5543; RRID: AB_571049 Mouse anti-S6 Cell Signaling Technology Cat#2317; RRID: AB_2238583 Rabbit anti-Phospho-S6 Cell Signaling Technology Cat#2211S; RRID: AB_331679 IRDye® 800CW Donkey anti-Mouse IgG Secondary Antibody LICORbio Cat#926–32212; RRID: AB_621847 IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody LICORbio Cat#926–68070; RRID: AB_10956588 Chemicals, peptides, and recombinant proteins Rapamycin Cedarlane Cat#13346-10 Accutase STEMCELL Technologies Cat#07922 Hoescht 33342 Enzo Cat#ENZ-52401 DMSO Sigma Cat#D2438 hESC matrigel Corning Cat#354277 DMEM-F12 Gibco Cat#11320-033 Neurobasal Gibco Cat#21103-049 N2 Gibco Cat#17502-048 B27 Gibco Cat#17504-044 MEM Non-Essential Amino Acids Solution (100X) Gibco Cat#11140-050 SB 431542 STEMCELL Technologies Cat#72234 LDN 193189 (2HCl) STEMCELL Technologies Cat#72147 mTeSR plus STEMCELL Technologies Cat#100-0276 HBSS Gibco Cat#14175-095 ReLeSR STEMCELL Technologies Cat#100-0483 ROCK inhibitor STEMCELL Technologies Cat#72304 Prolong antifade ThermoFisher Cat#P36984 Trypan blue STEMCELL Technologies Cat#07050 Cultrex BioTechne Cat#BME001-10 Glutamax Gibco Cat#35050-061 Pierce Protease Inhibitor Tablets ThermoFisher Cat#A32963 Phosphatase Inhibitor Cocktail V Millipore Sigma Cat#524629 RIPA Buffer Sigma-Aldrich Cat#R0278 OneTaq Quick-Load 2X Master Mix New England Biolabs Cat#M0486 PmeI New England Biolabs Cat#R0560 Cutsmart Buffer New England Biolabs Cat#B6004S 10× BlueJuice Gel Loading Buffer Thermo Fisher Cat10816015 Critical commercial assays PowerUp SYBR Green Master Mix Applied Biosystems Cat#A25741 Quick-DNA/RNA Microprep Plus Kit Zymo Research Cat#D7005 MycoFluor Mycoplasma Detection Kit Invitrogen Cat#M7006 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368814 (Continued on next page) e1 Cell Reports Methods 5, 101177, October 20, 2025 OPEN ACCESS .. Human PSC lines used in this study were 168 id2 (iPSC, male) and H1 (hESC, male; WiCell WA01).

    Article Title: High-throughput bioprinting to produce micropatterned neuroepithelial tissues and model TSC2-deficient brain malformations
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-PAX6 Proteintech Group Cat#12323-1-AP; RRID: AB_2159695 Rabbit anti-Ki-67 Proteintech Group Cat#27309-1-AP: RRID: AB_2756525 Mouse anti-ZO-1 Invitrogen Cat#33–9100; RRID: AB_2533147 Mouse anti-β-Tubulin III STEMCELL Technologies Cat# 60100 Mouse anti-N-cad Cell Signaling Cat#14215; RRID: AB_2798427 Chicken anti-MAP2 Millipore Sigma Cat#AB5543; RRID: AB_571049 Mouse anti-S6 Cell Signaling Technology Cat#2317; RRID: AB_2238583 Rabbit anti-Phospho-S6 Cell Signaling Technology Cat#2211S; RRID: AB_331679 IRDye® 800CW Donkey anti-Mouse IgG Secondary Antibody LICORbio Cat#926–32212; RRID: AB_621847 IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody LICORbio Cat#926–68070; RRID: AB_10956588 Chemicals, peptides, and recombinant proteins Rapamycin Cedarlane Cat#13346-10 Accutase STEMCELL Technologies Cat#07922 Hoescht 33342 Enzo Cat#ENZ-52401 DMSO Sigma Cat#D2438 hESC matrigel Corning Cat#354277 DMEM-F12 Gibco Cat#11320-033 Neurobasal Gibco Cat#21103-049 N2 Gibco Cat#17502-048 B27 Gibco Cat#17504-044 MEM Non-Essential Amino Acids Solution (100X) Gibco Cat#11140-050 SB 431542 STEMCELL Technologies Cat#72234 LDN 193189 (2HCl) STEMCELL Technologies Cat#72147 mTeSR plus STEMCELL Technologies Cat#100-0276 HBSS Gibco Cat#14175-095 ReLeSR STEMCELL Technologies Cat#100-0483 ROCK inhibitor STEMCELL Technologies Cat#72304 Prolong antifade ThermoFisher Cat#P36984 Trypan blue STEMCELL Technologies Cat#07050 Cultrex BioTechne Cat#BME001-10 Glutamax Gibco Cat#35050-061 Pierce Protease Inhibitor Tablets ThermoFisher Cat#A32963 Phosphatase Inhibitor Cocktail V Millipore Sigma Cat#524629 RIPA Buffer Sigma-Aldrich Cat#R0278 OneTaq Quick-Load 2X Master Mix New England Biolabs Cat#M0486 PmeI New England Biolabs Cat#R0560 Cutsmart Buffer New England Biolabs Cat#B6004S 10× BlueJuice Gel Loading Buffer Thermo Fisher Cat10816015 Critical commercial assays PowerUp SYBR Green Master Mix Applied Biosystems Cat#A25741 Quick-DNA/RNA Microprep Plus Kit Zymo Research Cat#D7005 MycoFluor Mycoplasma Detection Kit Invitrogen Cat#M7006 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368814 (Continued on next page) e1 Cell Reports Methods 5, 101177, October 20, 2025 Article ll OPEN ACCESS .. Human PSC lines used in this study were 168 id2 (iPSC, male) and H1 (hESC, male; WiCell WA01).



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